Hi folks!
Putting together a farewell gift for a work colleague in the field of Histopathology with an interest in guitars and Blues.
Stock standard ST-1 kit stained with Haematoxylin (Hx). Hx is a basic dye used to stain the acidic structures of tissue blue and is often simplistically said to stain the cellular nucleus. Hx is used in conjunction with a pink acidic counter stain Eosin to stain basic structures and provide contrast to the Hx. This is referred to as a H+E stain. Now, before anyone wants to tell me that Hx stains the nucleus 'purple', have a look at your slide after it has been 'blued' in alkaline solution before counterstaining with Eosin.
For the stain nerds here, this is what Wikipedia has to say about Hx.
"Haematoxylin or hematoxylin (/ˌhiːməˈtɒksɪlɪn/), also called natural black 1 or C.I. 75290, is a compound extracted from heartwood of the logwood tree (Haematoxylum campechianum)[1][2] with a chemical formula of C16H14O6. This naturally derived dye has been used as a histologic stain, as an ink[3][4][5][6] and as a dye in the textile and leather industry.[7][8] As a dye, haematoxylin has been called palo de Campeche,[8] logwood extract,[7] bluewood[9] and blackwood.[9] In histology, haematoxylin staining is commonly followed by counterstaining with eosin.[1][10][11] When paired, this staining procedure is known as H&E staining and is one of the most commonly used combinations in histology.[1][7][12][13][14] In addition to its use in the H&E stain, haematoxylin is also a component of the Papanicolaou stain (or Pap stain) which is widely used in the study of cytology specimens.[1][14]"
BTW, STATocaster is not a misspelling, it is a reference to urgency, 'STAT' which is relevant to our field of work.
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Originally Posted by DarkMark
