Thanx Stan, those two Basses are my best builds to date.
I have never been able to part with the Violin Bass... she is perfect for playing the Blues
With the origional question, yes, IMHO, different nut materials will make the tone change.
But it may be one of those great unanswered questions... get ten Guitarists to play the same instrument with different nut combos and half of them will pick the difference between a bone and brass nut, the other five wont.
It is all about how we hear tone and also the individual tonal preference comes into play.
We could go on forever, but maybe the answer you seek will come with experimentation.
Grab yourself a brass nut, try it out then swap it for a bone then synthetic one.
Who knows? you may be surprized as to what you hear, and with each Guitar the results will be different as there are so many different factors that come into play..
Timber, string through body or bridge, headstock size and shape, the list goes on...
But, at the end of the day, I will always maintain that a natural material such as bone will always improve the tone, regardless of what bridge.
It seems to my ear that a bone nut balances out the metallic tone of the bridge and gives the Guitar a more balanced and warmer tone.