Absolutely but it is always last step and I do it after the ears have had a break, I have a constant battle with band mates who insist that the only way to listen is loud with the bass pumped which is not only tiring but covers issues.
Should add that editing and mixing should be treated like an office job with a break every hour, give the ears, eyes and neck a rest, if you know what you are looking for and know your plugins the mastering step should be pretty quick